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Fluorochrome 神经元逆行示踪研究,荧光金专业供应商
时间:2020-03-23作者:博蕾德来源:博蕾德关注:
Fluorochrome公司是专业生产荧光金及荧光金抗体的专业高科技公司。公司核心产品荧光金于1985年推向市场,被广泛应用于神经元逆行示踪研究。荧光金在紫外线激发下发金黄色光,非常灵敏,不仅能标记胞浆,而且能很好地显示树突分枝,但是细胞核和核仁不染色;在胞体内分解慢,甚至在注射后存活2月标记强度仍无明显变化;比较耐紫外线的照射,褪色比较慢;可以经受许多组织学染色处理,因而可以和HRP、免疫组织化学等方法结合。Fluorochrome公司还提供荧光金抗体及红色荧光金,扩大了其应用范围。
Fluoro-GoldTM 荧光金的优势:
1) 极其敏感和可靠的逆行示踪剂
2)
不会被未损害的通道纤维占据
3)细胞外无荧光渗漏,不易扩散
4)保质期长,荧光存在时间广从1天到1个月
5)显色效果清楚、逼真
6)安全且易操作
7)可压力注射或电子透入动物体
8)可以经受多种组织学染色处理,因而可与其它常规神经学检测技术免疫荧光、免疫组化、射线自显迹法、HRP组织化学及其他荧光逆行示踪剂结合使用。
产品应用实例:
保存:
干粉,4℃避光保存,保质期可超过1年。液体,4℃避光保存,保质期6个月以上。
稀释:荧光金可用双蒸水或0.9%生理盐水或0.2M的中性磷酸盐缓冲液溶解。
订购信息:
品牌 产品名称 规格
Fluorochrome Fluoro-Gold 20mg(现货)
Fluorochrome Fluoro-Gold 50mg
Fluorochrome Fluoro-Gold 100mg
Fluorochrome Fluoro-Gold 150mg
Fluorochrome Fluoro-Gold 200mg
Fluorochrome Antibody to Fluoro-Gold 0.1ml×1
Fluorochrome Antibody to Fluoro-Gold 0.1ml×2
Fluorochrome Antibody to Fluoro-Gold 0.1ml×3
Fluorochrome Fluoro-Ruby(红色荧光金) 30mg
应用文献:
  • A.P.Tosolini,et al.Spatial characterization of the motor neuron columns supplying the rat forelimb.Neuroscience,Volume 200, 3 January 2012, Pages 19-30
  • E.van Asselt,et al.Spinal neurons in the zebrafish labeled with Fluoro-gold and wheat-germ agglutinin.Neuroscience Volume 43, Issues 2–3, 1991, Pages 611-622
  • Lisa A. Catapano,et al.Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold. Neural Stem Cells pp 353-359
  • LNovikova,et al.Persistent neuronal labeling by retrograde fluorescent tracers: a comparison between Fast Blue, Fluoro-Gold and various dextran conjugates.Journal of Neuroscience Methods Volume 74, Issue 1, 6 June 1997, Pages 9-15
  • LC Schmued,et al.Fluoro-Gold: a new fluorescent retrograde axonal tracer with numerous unique properties.Brain Research Volume 377, Issue 1, 2 July 1986, Pages 147-154
  • Junjie Yang,et al.Flow cytometry for quantification of retrogradely labeled retinal ganglion cells by Fluoro-Gold.Journal Current Eye Research Volume 21, 2000 - Issue 6
  • AnnaPuigdellı́vol-Sánchez,et al.On the use of fast blue, fluoro-gold and diamidino yellow for retrograde tracing after peripheral nerve injury: uptake, fading, dye interactions, and toxicity.Journal of Neuroscience Methods Volume 115, Issue 2, 15 April 2002, Pages 115-127
  • Estudo comparativo entre enxerto autógeno e enxerto muscular coberto com tubo de veia autógeno em nervos tibiais de ratos wistar, utilizando fluoro-gold® como marcador neuronal,Acta ortop. bras. vol.15 no.2 São Paulo 2007
  • Arthur HNeufeld,et al.The inherent, age-dependent loss of retinal ganglion cells is related to the lifespan of the species.Neurobiology of Aging.Volume 24, Issue 1, January–February 2003, Pages 167-172
  • JavierRuiz-Ederra,et al.Mouse model of sustained elevation in intraocular pressure produced by episcleral vein occlusion.Experimental Eye Research Volume 82, Issue 5, May 2006, Pages 879-884