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Proteinase K (PROK) Recombinant Protein
Proteinase K (PROK) Recombinant Protein
- 中文名称：
- Proteinase K (PROK) Recombinant Protein
- 英文名称：
- Proteinase K (PROK) Recombinant Protein
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA233469
- 规格：
- 1 kg|2x1 kg|3x1 kg|4x1 kg|5x1 kg
- 保存建议：
- Aliquot and store at<= -20°C. Avoid repeated freeze / thaw cycles
- 货期：
- 6-8周
- 纯度：
- 90% ± 5% by SDS-PAGE
- 产品形式：
- Liquid, dissolved in Tris-HCl, pH 7.5, 10%glycerol?0.02%NaN3
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Proteinase K is an extracellular serine endoproteinase produced by Tritirachium album Limber. Proteinase K belongs to the peptidase family S8. Its predominant cleavage site is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked ?-amino groups. Proteinase K is a broad-spectrum protease that functions at wide pH and temperature ranges. The optimum pH and temperature for the activity of this protease were approximately pH 7.5-8.0 and 55°C, respectively. In addition, 0.2-1% sodium dodecyl sulfate (SDS) and 10mM carbamide can activate its activity.
The combination of these unique features makes Proteinase K as an important tool in scientific research. Proteinase K can be used in the preparation of biological macromolecules by degrading the constitutive proteins of cells and removing nucleases such as DNases and RNases for RT-PCR. In nucleic acid vaccine and molecular identification, it is also widely used for degrading proteinaceous impurities during the isolation of nucleic acids from biological samples.
The latest launch of high quality of Proteinase K shows its high purity and high enzyme activity by biochemical platform testing. Through repeated inspection, all the data show our company produced Proteinase K without RNA, DNA, RNAse and DNAse residues.
