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Goat anti Chicken IgM (Fc specific), conjugated with FITC
Goat anti Chicken IgM (Fc specific), conjugated with FITC
- 中文名称:
- Goat anti Chicken IgM (Fc specific), conjugated with FITC
- 英文名称:
- Goat anti Chicken IgM (Fc specific), conjugated with FITC
- 品牌:
- AAA Biotech
- 品牌介绍:
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具,核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号:
- AAA77551
- 规格:
- 1 mL|2x1 mL|3x1 mL|4x1 mL|5x1 mL
- 保存建议:
- The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 degree C; prolonged storage at or below -20 degree C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 degree C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 degree C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Lyophilized at +4 degree C--at least 10 years. Reconstituted at or below -20 degree C--3-5 years. Reconstituted at +4 degree C--7 days
- 货期:
- 6-8周
- 纯度:
- Hyperimmune antisera with strong precipitating activity are selected for fractionation and purification of the IgG (7S) fraction containing the bulk of the defined antibody specificity. It IS free of other serum proteins as tested by immunoelectrophoresis
- 产品形式:
- FITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
- 免责声明:
- *本产品仅供科研实验使用,不得用于临床诊断。*
- 说明书:
The reactivity of the antiserum is directed to the Fc subunit of the IgM molecule, which expresses strict (class) specificity. In immunoelectrophoresis and radial in immunodiffusion using various antiserum concentrations against chicken serum, a single precipitin line has been obtained which shows a reaction of identity with the precipitin lines obtained with the purified IgM used as immunogens. It does not react with IgG, IgG/Fab fragments and IgA or any non-Ig protein in chicken serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunocytochemical and immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of chicken origin known to be of the IgM isotype in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40, depending on the method used.