-
Goat anti Rat IgE (Fc specific)
Goat anti Rat IgE (Fc specific)
- 中文名称：
- Goat anti Rat IgE (Fc specific)
- 英文名称：
- Goat anti Rat IgE (Fc specific)
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA77608
- 规格：
- 10 mg|2x10 mg|3x10 mg|4x10 mg|5x10 mg
- 保存建议：
- Handling:The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4 degree C; prolonged storage at or below -20 degree C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 degree C . Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4 degree C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Packing: Vial with 10 mg lyophilized IgG fraction.
Storage/Shelf life: Lyophilized at +4 degree C/at least 10 years
reconstituted at or below -20 degree C/3-5 years
reconstituted at +4 degree C/7 days
- 货期：
- 6-8周
- 纯度：
- Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.
- 产品形式：
- Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
The reactivity of the antiserum is directed to the Fc subunits of the IgE molecule which represents strict isotype (class) specificity. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against appropriate concentrations of IgE, a single precipitin line is obtained which shows a reaction of full identity with the precipitin line obtained with the used immunogen. It does not react with rat IgG, including all subclasses, IgA, IgM and IgG/Fab or any non-Ig protein in rat serum. As unlabelled secondary antibody for indirect detection of IgE in rat cell, tissue substrates and body fluids in immunofluorescence and immunoenzyme assay methods; for the production of immunoconjugates with a selected marker; to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier; as catching or detecting antibody reagent in non-isotopic assay methodology (e.g . ELISA) to identify and measure IgE in rat serum or other body fluid. When applied in any immunocytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established.
