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Human Anti-carbamylated Protein Antibody ELISA Kit
Human Anti-carbamylated Protein Antibody ELISA Kit
- 中文名称：
- Human Anti-carbamylated Protein Antibody ELISA Kit
- 英文名称：
- Human Anti-carbamylated Protein Antibody ELISA Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA27381
- 规格：
- 48-Strip-Wells|96-Strip-Wells|2x96-Strip-Wells|3x96-Strip-Wells|4x96-Strip-Wells
- 保存建议：
- 请按照说明书指示
- 货期：
- 6-8周
- 检测种属：
- Human
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Principle of the Assay: ACPA ELISA kit applies the competitive enzyme immunoassay technique utilizing Carbamylated Protein antigen and an ACPA-HRP conjugate. The assay sample and buffer are incubated together with ACPA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACPA concentration since ACPA from samples and ACPA-HRP conjugate compete for the Carbamylated Protein antigen binding site. Since the number of sites is limited, as more sites are occupied by ACPA from the sample, fewer sites are left to bind ACPA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACPA concentration in each sample is interpolated from this standard curve.
Intended Uses: This ACPA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ACPA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
