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Mouse Anti Glutamic Acid Decarboxylase 65 antibody ELISA Kit
Mouse Anti Glutamic Acid Decarboxylase 65 antibody ELISA Kit
- 中文名称：
- Mouse Anti Glutamic Acid Decarboxylase 65 antibody ELISA Kit
- 英文名称：
- Mouse Anti Glutamic Acid Decarboxylase 65 antibody ELISA Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA27407
- 规格：
- 48-Strip-Wells|96-Strip-Wells|2x96-Strip-Wells|3x96-Strip-Wells|4x96-Strip-Wells
- 保存建议：
- 请按照说明书指示
- 货期：
- 6-8周
- 检测种属：
- Mouse
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Intended Uses: This GAD65Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse GAD65Ab. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: GAD65Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing Glutamic Acid Decarboxylase 65 antigen and an GAD65Ab-HRP conjugate. The assay sample and buffer are incubated together with GAD65Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GAD65Ab concentration since GAD65Ab from samples and GAD65Ab-HRP conjugate compete for the Glutamic Acid Decarboxylase 65 antigen binding site. Since the number of sites is limited, as more sites are occupied by GAD65Ab from the sample, fewer sites are left to bind GAD65Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GAD65Ab concentration in each sample is interpolated from this standard curve.
