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Mouse Pleiotrophin ELISA Kit
Mouse Pleiotrophin ELISA Kit
- 中文名称：
- Mouse Pleiotrophin ELISA Kit
- 英文名称：
- Mouse Pleiotrophin ELISA Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA101230
- 规格：
- 48-Strip-Wells|96-Strip-Wells|2x96-Strip-Wells|5x96-Strip-Wells|10x96-Strip-Wells
- 保存建议：
- 将整套工具短期储存在2-8摄氏度的环境中。如果要长期保存，请将微孔板和标准品保存在-20摄氏度，而剩余的试剂可以保存在2-8摄氏度
- 货期：
- 6-8周
- 检测种属：
- Mouse
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Background: Pleiotrophin (PTN) and midkine (MK) comprise a family of structurally related, developmentally regulated genes. Cells reported to express PTN include osteoblasts, chondrocytes, fibroblasts, astrocytes, oligodendroglia, Schwann cells, neurons, pituicytes and Leydig cells. PTN is a heparin-binding growth factor that functions as a weak mitogen and promotes neurite outgrowth from embryonic brain neurons. Diseases associated with PTN include Nasal Cavity Olfactory Neuroblastoma and Benign Meningioma.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti PTN antibody was pre-coated onto the 96-well plate. The biotin conjugated anti PTN antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with PTN conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of PTN in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
