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Mouse Glutamate decarboxylase autoantibody ELISA Kit
Mouse Glutamate decarboxylase autoantibody ELISA Kit
- 中文名称:
- Mouse Glutamate decarboxylase autoantibody ELISA Kit
- 英文名称:
- Mouse Glutamate decarboxylase autoantibody ELISA Kit
- 品牌:
- AAA Biotech
- 品牌介绍:
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具,核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号:
- AAA101694
- 规格:
- 48-Strip-Wells|96-Strip-Wells|2x96-Strip-Wells|5x96-Strip-Wells|10x96-Strip-Wells
- 保存建议:
- 将整套工具短期储存在2-8摄氏度的环境中。如果要长期保存,请将微孔板和标准品保存在-20摄氏度,而剩余的试剂可以保存在2-8摄氏度
- 货期:
- 6-8周
- 检测种属:
- Mouse
- 免责声明:
- *本产品仅供科研实验使用,不得用于临床诊断。*
- 说明书:
Principle of the Assay||This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antigen specific to Mouse GAD-Ab. Standards or samples are then added to the appropriate micro ELISA plate wells and combined to the specific antigen. Then a biotinylated detection antigen specific for Mouse GAD-Ab and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GAD-Ab, biotinylated detection antigen and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turn yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse GAD-Ab. You can calculate the concentration of Mouse GAD-Ab in the samples by comparing the OD of the samples to the standard curve.