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Rat DNA damage-inducible transcript 3 protein ELISA Kit
Rat DNA damage-inducible transcript 3 protein ELISA Kit
- 中文名称:
- Rat DNA damage-inducible transcript 3 protein ELISA Kit
- 英文名称:
- Rat DNA damage-inducible transcript 3 protein ELISA Kit
- 品牌:
- AAA Biotech
- 品牌介绍:
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具,核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号:
- AAA101710
- 规格:
- 48-Strip-Wells|96-Strip-Wells|2x96-Strip-Wells|5x96-Strip-Wells|10x96-Strip-Wells
- 保存建议:
- 将整套工具短期储存在2-8摄氏度的环境中。如果要长期保存,请将微孔板和标准品保存在-20摄氏度,而剩余的试剂可以保存在2-8摄氏度
- 货期:
- 6-8周
- 检测种属:
- Rat
- 免责声明:
- *本产品仅供科研实验使用,不得用于临床诊断。*
- 说明书:
Principle of the Assay||This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Ddit3. During the reaction,Rat Ddit3 in the sample or standard competes with a fixed amount of biotin-labeled Rat Ddit3 for sites on a pre-coated Monoclonal antibody specific to Rat Ddit3. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat Ddit3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.