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Rat C-Reactive Protein ELISA Kit
Rat C-Reactive Protein ELISA Kit
- 中文名称：
- Rat C-Reactive Protein ELISA Kit
- 英文名称：
- Rat C-Reactive Protein ELISA Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA105513
- 规格：
- 96 Tests|2x96 Tests|3x96 Tests|4x96 Tests|5x96 Tests
- 保存建议：
- Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
- 货期：
- 6-8周
- 检测种属：
- Rat
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Principle of the Assay: The AAA Biotech' Rat C-Reactive Protein ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat C-Reactive Protein in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Rat C-Reactive Protein coated on a 96-well plate. Standards and samples are pipetted into the wells and C-Reactive Protein present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat C-Reactive Protein antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of C-Reactive Protein bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: C Reactive Protein (CRP) is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is composed of 5 identical, 21,500-molecular weight subunits. CRP mediates activities associated with preimmune nonspecific host resistance. CRP shows the strongest association with cardiovascular events. It is detectable on the surface of about 4% of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver.
