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Human NOV ELISA Kit
Human NOV ELISA Kit
- 中文名称:
- Human NOV ELISA Kit
- 英文名称:
- Human NOV ELISA Kit
- 品牌:
- AAA Biotech
- 品牌介绍:
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具,核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号:
- AAA105541
- 规格:
- 96 Tests|2x96 Tests|3x96 Tests|4x96 Tests|5x96 Tests
- 保存建议:
- Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
- 货期:
- 6-8周
- 检测种属:
- Human
- 免责声明:
- *本产品仅供科研实验使用,不得用于临床诊断。*
- 说明书:
Principle of the assay: The Human NOV ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human NOV in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human NOV coated on a 96-well plate. Standards and samples are pipetted into the wells and NOV present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human NOV antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of NOV bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background: NOV (nephroblastoma overexpressed), also known as CCN3, is a matricellular protein that in humans is encoded by the NOV gene. The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. This gene is mapped to 8q24.12. NOV is a potentially useful marker for the identification of adrenal gland diseases, malignant adrenocortical tumors, multiple sclerosis and so on. Moreover, reduced expression of NOV in ACTs may play an important role in the process of childhood ACT tumorigenesis. Though studying, it identified Nov as a regulator of human hematopoietic stem or progenitor cells.