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Human CCL3 ELISA Kit
Human CCL3 ELISA Kit
- 中文名称：
- Human CCL3 ELISA Kit
- 英文名称：
- Human CCL3 ELISA Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA105561
- 规格：
- 96 Tests|2x96 Tests|3x96 Tests|4x96 Tests|5x96 Tests
- 保存建议：
- Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
- 货期：
- 6-8周
- 检测种属：
- Human
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Principle of the Assay: The Human CCL3 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CCL3 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human CCL3 coated on a 96-well plate. Standards and samples are pipetted into the wells and CCL3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CCL3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CCL3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: Macrophage inflammatory protein-1 alpha (MIP-1 alpha), also called CCL3, LD78. The cDNA for MIP-1 alpha predicts a mature peptide of 69 amino acids with a molecular mass of 7,889 daltons. LD78 is a member of a newly identified superfamily of small inducible proteins involved in inflammatory responses, wound healing and tumorigenesis. MIP-1 alpha is a chemokine that has pro-inflammatory and stem cell inhibitory activities in vitro. It constitutes an important second signal for mast cell degranulation in the conjunctiva in vivo and consequently for acute-phase disease.
