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Human Vitamin C ELISA Kit
Human Vitamin C ELISA Kit
- 中文名称：
- Human Vitamin C ELISA Kit
- 英文名称：
- Human Vitamin C ELISA Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA124081
- 规格：
- 96-Strip-Wells|2x96-Strip-Wells
- 保存建议：
- 在2至8摄氏度下储存6个月。
- 货期：
- 6-8周
- 检测种属：
- Human
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Principle of the Assay: This kit is a Enzyme-Linked Immunosorbent Assay (ELISA). Add samples to the pre-coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin-HRP is then added and then incubate. Unbound avidin-HRP is washed away during a washing step. TMB Substrate is then added and color develops. The reaction is stopped by addition of acidic stop solution and color changes into yellow that can be measured at 450 nm. The intensity of the color developed is inversely proportional to the concentration of VC in the sample. The concentration of VC in the sample is then determined by comparing the O.D. of the samples to the standard curve.
Intended Uses: This competitive ELISA kit is for the quantification of Vitamin C(also known as VC) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.
