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Rat JAM-C PicoKine ELISA Kit
Rat JAM-C PicoKine ELISA Kit
- 中文名称：
- Rat JAM-C PicoKine ELISA Kit
- 英文名称：
- Rat JAM-C PicoKine ELISA Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA125070
- 规格：
- 96-Strip-Wells|2x96-Strip-Wells|5x96-Strip-Wells
- 保存建议：
- 请按照说明书指示
- 货期：
- 6-8周
- 检测种属：
- Rat
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Principle of the Assay: The Picokine Rat Il6st Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat Il6st with a 96-well strip plate that is pre-coated with antibody specific for Il6st. The detection antibody is a biotinylated antibody specific for Il6st. The capture antibody is A monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. The kit contains recombinant Rat Il6st with immunogen: Expression system for standard: NSO, Immunogen sequence: Q23-E618. The kit is analytically validated with ready to use reagents. To measure Rat Il6st, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Rat Il6st in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Il6st in the sample!!Background/Introduction: GP130(Glycoprotein 130) also known as IL6ST or CD130, is a transmembrane protein which is the founding member of the class of all cytokine receptors. The GP130 gene is mapped on 5q11.2. Gp130 showed an apparent molecular mass of 130 kD by SDS-PAGE. Gp130 is involved in the formation of high-affinity IL6-binding sites and in IL6 signal transduction. Sgp130 has a role in modulating signals transduced by membranebound gp130. Gp130 is an important part of many different types of signaling complexes. Gp130 molecules with mutations in either of these segments could not transduce a growth signal.
