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Human CPA2 PicoKine ELISA Kit
Human CPA2 PicoKine ELISA Kit
- 中文名称：
- Human CPA2 PicoKine ELISA Kit
- 英文名称：
- Human CPA2 PicoKine ELISA Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA125074
- 规格：
- 96-Strip-Wells|2x96-Strip-Wells|5x96-Strip-Wells
- 保存建议：
- 请按照说明书指示
- 货期：
- 6-8周
- 检测种属：
- Human
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Principle of the Assay: The Picokine Human COMP Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human COMP with a 96-well strip plate that is pre-coated with antibody specific for COMP. The detection antibody is a biotinylated antibody specific for COMP. The capture antibody is polyclonal antibody from goat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human COMP with immunogen: Expression system for standard: NSO; Immunogen sequence: Q21-A757. The kit is analytically validated with ready to use reagents. To measure Human COMP, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human COMP in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human COMP in the sample!!Background/Introduction: Cartilage oligomeric matrix protein is a protein that in humans is encoded by the COMP gene. The sequences of rat and bovine COMP indicate that it is a member of the thrombospondin gene family. By Southern blot analysis of a somatic cell hybrid DNA panel and by isotopic in situ hybridization, human COMP gene was mapped to 19p13.1, and the murine COMP gene was mapped to the central region of mouse chromosome 8 by use of an interspecific backcross mapping panel. COMP is a marker of cartilage turnover.
