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Human Serpin A12 PicoKine ELISA Kit
Human Serpin A12 PicoKine ELISA Kit
- 中文名称：
- Human Serpin A12 PicoKine ELISA Kit
- 英文名称：
- Human Serpin A12 PicoKine ELISA Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA125077
- 规格：
- 96-Strip-Wells|2x96-Strip-Wells|5x96-Strip-Wells
- 保存建议：
- Store at 4 degree C for 6 months. Store at -20 degree C for 12 months.
Avoid multiple freeze-thaw cycles.
- 货期：
- 6-8周
- 检测种属：
- HumanThere is no detectable cross-reactivity with other relevant proteins.
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Principle of the Assay: The Picokine Human Serpin A1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human Serpin A1 with a 96-well strip plate that is pre-coated with antibody specific for Serpin A1. The detection antibody is a biotinylated antibody specific for Serpin A1. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human Serpin A1 with immunogen: Expression system for standard: NSO,E25-K418. The kit is analytically validated with ready to use reagents. To measure Human Serpin A1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human Serpin A1 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human Serpin A1 in the sample!!Background/Introduction: Alpha-1-antitrypsin or alpha1-antitrypsin (A1AT, A1A, or AAT) is a protein belonging to the serpin superfamily. It is encoded in humans by the SERPINA1 gene. The gene is located on the long arm of the fourteenth chromosome (14q32.1).The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene.Like all serine protease inhibitors, A1AT has a characteristic secondary structure of beta sheets and alpha helices. Mutations in these areas can lead to non-functional proteins that can polymerise and accumulate in the liver(infantile hepatic cirrhosis).
