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Hamster Putative phospholipase B-like 2 (PLBD2) ELISA Kit
Hamster Putative phospholipase B-like 2 (PLBD2) ELISA Kit
- 中文名称：
- Hamster Putative phospholipase B-like 2 (PLBD2) ELISA Kit
- 英文名称：
- Hamster Putative phospholipase B-like 2 (PLBD2) ELISA Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA13241
- 规格：
- 48 Tests|96 Tests|2x96 Tests|3x96 Tests|4x96 Tests
- 保存建议：
- 储存在2-8摄氏度
- 货期：
- 6-8周
- 检测种属：
- Hamster
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Most soluble lysosomal proteins carry Man6P (mannose 6-phosphate), a specific carbohydrate marker that enables their binding to cellular MPRs (Man6P receptors) and their subsequent targeting towards the lysosome.
This characteristic was exploited to identify novel soluble lysosomal proteins by proteomic analysis of Man6P proteins purified from a human cell line. The presence of Man6P sugars was confirmed by an MPR overlay experiment, which showed the direct and Man6P-dependent interaction between p76 and the MPR. The presence of six N-glycosylation sites was validated by progressive peptide-N-glycosidase F deglycosylation. Experiments using N- and C-termini directed anti-p76 antibodies provided insights into p76 maturation.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PLBD2 in samples. An antibody specific for PLBD2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLBD2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLBD2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLBD2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
