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Trastuzumab (Herceptin) PK
Trastuzumab (Herceptin) PK
- 中文名称：
- Trastuzumab (Herceptin) PK
- 英文名称：
- Trastuzumab (Herceptin) PK
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA13589
- 规格：
- 96 Wells|2x96 Wells|3x96 Wells|4x96 Wells|5x96 Wells
- 保存建议：
- Store kit components at -20 degree C unless specified otherwise. Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
- 货期：
- 6-8周
- 检测种属：
- N/A
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Background: Trastuzumab (Herceptin) is a humanized monoclonal antibody used in the treatment of HER2- positive cancers. Trastuzumab binds to domain IV of the extracellular domain of the HER2/neu receptor. Cells treated with Trastuzumab undergo cell cycle arrest during the G1 phase. It has been suggested that trastuzumab does not alter HER-2 expression, but decreases activation of AKT. The Trastuzumab ELISA kit is designed to measure Trastuzumab with high specificity and enhanced sensitivity. The assay design utilizes a pair of antibodies that allows the detection of the whole Trastuzumab molecule in biological matrices.
Principle of the Assay: This assay employs the sandwich enzyme immunoassay technique. Anti- Trastuzumab is coated onto a 96 well microplate. Calibrator, quality control samples (if desired) and test samples are pipetted into the appropriate wells. Trastuzumab present in biological matrices is bound by the immobilized anti- Trastuzumab antibody. After washing away any unbound substances, enzyme linked antiTrastuzumab antibody is added to the wells. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of Trastuzumab present in test samples. The color development is stopped and the intensity of the color is measured.
