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Human Interleukin 12A (IL12A) ELISA Kit
Human Interleukin 12A (IL12A) ELISA Kit
- 中文名称:
- Human Interleukin 12A (IL12A) ELISA Kit
- 英文名称:
- Human Interleukin 12A (IL12A) ELISA Kit
- 品牌:
- AAA Biotech
- 品牌介绍:
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具,核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号:
- AAA136047
- 规格:
- 24-Strip-Wells|48-Strip-Wells|96-Strip-Wells|2x96-Strip-Wells|5x96-Strip-Wells
- 保存建议:
- 此类产品特殊,请按照说明书指示
- 货期:
- 6-8周
- 检测种属:
- Human
- 免责声明:
- *本产品仅供科研实验使用,不得用于临床诊断。*
- 说明书:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 12A (IL12A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 12A (IL12A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 12A (IL12A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 12A (IL12A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.