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BrdU Cell Proliferation
BrdU Cell Proliferation
- 中文名称：
- BrdU Cell Proliferation
- 英文名称：
- BrdU Cell Proliferation
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA13607
- 规格：
- 1000 Tests|2x1000 Tests|3x1000 Tests|4x1000 Tests|5x1000 Tests
- 保存建议：
- Store kit at -20 degree C until first use. Store at 4-8 degree C thereafter.
- 货期：
- 6-8周
- 检测种属：
- BrdU Incorporated Cells
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Background: The BrdU Cell Proliferation Assay Kit is a non-isotopic enzyme immunoassay for the quantification of DNA synthesis and cell proliferation. Evaluation of cell cycle progression is essential for investigations in many scientific fields. Measurement of [3H] thymidine incorporation as cells enter S phase has long been the traditional method for the detection of cell proliferation. Subsequent quantification of [3H] thymidine is performed by scintillation counting or autoradiography. This technology is slow, labor intensive and has several limitations including the handling and disposal of radioisotopes and the necessity of expensive equipment. A well-established alternative to [3H] thymidine uptake has been demonstrated by numerous investigators. In these methods bromodeoxyuridine (BrdU), a thymidine analog, replaces [3H] thymidine. BrdU is incorporated into newly synthesized DNA strands of actively proliferating cells. Following partial denaturation of double stranded DNA, BrdU is detected immunochemically allowing the assessment of the population of cells which are actively synthesizing DNA. BrdU Cell Proliferation Assay Kit involves incorporation of BrdU Reagent into cells cultured in microtiter plates using the cell layer as the solid phase. The resultant assay is, rapid, easy to perform and applicable to high sample throughput. In addition to evaluation of cell proliferation, information such as cell number, morphology and analysis of cellular antigens can be obtained from a single culture.
Principle of the Assay: BrdU Cell Proliferation Assay Kit involves incorporation of BrdU Reagent into cells cultured in microtiter plates using the cell layer as the solid phase. During the final 2 to 24 hours of culture 1X BrdU Reagent is added to wells of the microtiter plate. BrdU Reagent will be incorporated into the DNA of dividing cells. To enable antibody binding to the incorporated BrdU, cells must be fixed, permeabilized and the DNA denatured. This is all done in one step by treatment with Fixative/Denaturing Solution. Prediluted Anti-BrdU Detector Antibody is pipetted into the wells and allowed to incubate for one hour, during which time it binds to any incorporated BrdU Reagent. Unbound Prediluted Anti-BrdU Detector Antibody is washed away and 1x Peroxidase Goat anti-Mouse IgG is added, which binds to the Prediluted Anti-BrdU Detector Antibody. The 1x Peroxidase Goat anti-Mouse IgG catalyzes the conversion of the TMB Substrate (chromogenic substrate tetra-methylbenzidine) from a colorless solution to a blue solution (or yellow after the addition of ELISA Stop Solution), the intensity of which is proportional to the amount of incorporated BrdU Reagent in the cells. The colored reaction product is quantified using a spectrophotometer.
Intended Uses: The BrdU Cell Proliferation Assay Kit is a non-isotopic immunoassay for the quantitation of bromodeoxyuridine incorporation into newly synthesized DNA of actively proliferating cells.
