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Human Anti-COVID-19 Nucleocapsid Protein (NP) IgG ELISA Kit
Human Anti-COVID-19 Nucleocapsid Protein (NP) IgG ELISA Kit
- 中文名称：
- Human Anti-COVID-19 Nucleocapsid Protein (NP) IgG ELISA Kit
- 英文名称：
- Human Anti-COVID-19 Nucleocapsid Protein (NP) IgG ELISA Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA13612
- 规格：
- 96 Wells|2x96 Wells|3x96 Wells|4x96 Wells|5x96 Wells
- 保存建议：
- The kit should be stored at 2-8 degree C upon receipt.
- 货期：
- 6-8周
- 检测种属：
- Human
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Principle of the Assay: The SARS-CoV-2 NP IgG ELISA kit is a two-step incubation immunoassay kit. Recombinant nucleocapsid protein (NP) of SARS-CoV-2 pre-coated onto the polystyrene microwell strips can specifically recognize anti- NP antibodies in human serum or plasma specimens. After a 1-hour incubation, anti-NP antibodies are captured by immobilized NP protein while the unbound components are washed away. Afterwards, a detection solution containing HRP-conjugated anti- human IgG is added for another 1-hour incubation, wherein HRP- conjugated anti-human IgG binds to the IgG class antibodies previously bound to NP protein on the plate. After removal of nonspecific bindings, a HRP substrate solution containing 3,3?,5,5?-Tetramethylbenzidine (TMB) is added, resulting in the formation of a blue color. Color reaction is stopped by 2M H2SO4, transforming the blue color to yellow signals, which is quantified by an absorbance microplate reader at 450nm. The color intensity is proportional to the amount of anti-NP antibodies captured inside the wells.
Intended Uses: SARS-CoV-2 NP IgG ELISA kit is a highly sensitive and specific enzyme-linked immunosorbent assay (ELISA) for the detection and qualitative measurement of IgG class antibodies against the nucleocapsid protein (NP) of SARS-CoV-2 virus in human blood.
