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High-sensitive SARS-CoV-2 S1 IgG ELISA Kit
High-sensitive SARS-CoV-2 S1 IgG ELISA Kit
- 中文名称:
- High-sensitive SARS-CoV-2 S1 IgG ELISA Kit
- 英文名称:
- High-sensitive SARS-CoV-2 S1 IgG ELISA Kit
- 品牌:
- AAA Biotech
- 品牌介绍:
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具,核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号:
- AAA13615
- 规格:
- 96 Wells|2x96 Wells|3x96 Wells|4x96 Wells|5x96 Wells
- 保存建议:
- 请按照说明书指示
- 货期:
- 6-8周
- 检测种属:
- N/A
- 免责声明:
- *本产品仅供科研实验使用,不得用于临床诊断。*
- 说明书:
Background: SARS-CoV-2 is an enveloped virus with a positive-sense RNA genome and a nucleocapsid of helical symmetry. The SARS-CoV-2 entry into host cells is mediated by the transmembrane spike (S) glycoprotein.
Principle of the Assay: 96-well plates are coated with SARS-CoV-2 S1 protein that captures IgG antibodies against SARS- CoV-2 S1 protein in the sample. After washing away unbound materials, captured IgG against SARS-CoV-2 S1 protein is detected by anti-human IgG polyclonal antibodies conjugated with horse radish peroxidase (HRP). After washing step, the chromogenic substrate 3,3',5,5'- tetramethylbenzidine (TMB) is added. Color reaction is stopped by 2M H2SO4. The amount of anti- SARS- CoV-2 S1 antibodies captured inside the wells is proportional to the color density generated in the coupled oxidation-reduction reaction. The unknown sample concentration can be interpolated from a standard curve generated by Human anti-S1 monoclonal antibody (mAb).
Intended Uses: SARS-CoV-2 S1 protein IgG ELISA Kit is a highly sensitive and specific immunoassay for the precision detection and quantitative measurement of IgG class antibodies against the spike S1 protein of SARS-CoV-2 virus in human blood samples in vitro.