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SARS-CoV-2 Spike S1 IgA ELISAKit
SARS-CoV-2 Spike S1 IgA ELISAKit
- 中文名称：
- SARS-CoV-2 Spike S1 IgA ELISAKit
- 英文名称：
- SARS-CoV-2 Spike S1 IgA ELISAKit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA13619
- 规格：
- 96 Wells|2x96 Wells|3x96 Wells|4x96 Wells|5x96 Wells
- 保存建议：
- The kit should be stored at 2-8 degree C, and all reagents should be equilibrated to room temperature before use. Immediately after use remaining reagents should be returned to cold storage (2- 8 degree C). Expiry of the kit and reagents is stand on labels. Once opened, the strips may be stored at 2-8 degree C for up to one month.
- 货期：
- 6-8周
- 检测种属：
- N/A
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Background: SARS-CoV-2 is an enveloped virus with a positive-sense RNA genome and a nucleocapsid of helical symmetry. The SARS-CoV-2 entry into host cells is mediated by the transmembrane spike (S) glycoprotein, which is the main target of neutralizing antibodies upon infection and the focus of the therapeutic and vaccine design. S comprises two functional subunits responsible for binding to the host cell receptor (S1 subunit) and fusion of the viral and cellular membranes (S2 subunit). Immunoglobulin A (IgA) plays a crucial role in the immune function of mucous membranes. In the blood, IgA interacts with Fc receptor to initiate inflammatory responses.
Principle of the Assay: 96-well plates are coated with SARS-CoV-2 S1 protein that captures antibodies against SARS- CoV-2 S1 protein in the sample. After washing away unbound materials, captured IgA against SARS-CoV-2 S1 protein is detected by anti-human IgA monoclonal antibodies conjugated with horse radish peroxidase (HRP), which is specific to human IgA, with no cross-reaction with human IgG or IgM. After washing step, the chromogenic substrate 3,3',5,5'-tetramethylbenzidine (TMB) is added. Color reaction is stopped by 2M H2SO4. The amount of IgA class antibodies against SARS-CoV-2 S1 captured inside the wells is proportional to the color density generated in the coupled oxidation-reduction reaction.
Intended Uses: SARS-CoV-2 S1 IgA ELISA Kit is a highly sensitive and specific immunoassay developed for quantitative detection of IgA class antibodies against the S1 of SARS- CoV-2 in human blood.
