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High-sensitivity SARS-CoV-2 S1RBD IgG ELISA Kit
High-sensitivity SARS-CoV-2 S1RBD IgG ELISA Kit
- 中文名称：
- High-sensitivity SARS-CoV-2 S1RBD IgG ELISA Kit
- 英文名称：
- High-sensitivity SARS-CoV-2 S1RBD IgG ELISA Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA13620
- 规格：
- 96 Wells|2x96 Wells|3x96 Wells|4x96 Wells|5x96 Wells
- 保存建议：
- 请按照说明书指示
- 货期：
- 6-8周
- 检测种属：
- N/A
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Background: The spike protein (S) is an envelope-anchored protein that mediates the recognition and binding of SARSCoV-2 to host cells. S can be further cleaved by the host protease into two subunits called S1 and S2. S1 polypeptide contain a receptor binding domain (S1RBD) crucial for the specific recognition and interaction with the human receptor ACE2, which is the first and the most essential step for the virus infection.
Principle of the Assay: SARS-CoV-2 S1RBD IgG ELISA kit is a two-step incubation immunoassay kit. Recombinant spike protein S1 receptor- binding domain (S1RBD) of SARS-CoV-2 pre-coated onto the polystyrene microwell strips can specifically recognize anti-S1RBD antibodies in human serum or plasma specimen. After a 1-hour incubation, anti-S1RBD antibodies are captured by immobilized S1RBD protein while the unbound components are washed away. Afterwards, a detection solution containing HRP-conjugated anti-human IgG is added for another 1-hour incubation, wherein HRP-conjugated anti-human IgG binds to the IgG class antibodies previously bound to S1RBD protein on the plate. After removal of nonspecific bindings, a HRP substrate solution containing 3,3?,5,5?- Tetramethylbenzidine (TMB) is added, resulting in the formation of a blue color. Color reaction is stopped by 2M H2SO4, transforming the blue color to yellow signals, which is quantified by an absorbance microplate reader at 450nm. The color intensity is proportional to the amount of anti- S1RBP antibodies captured inside the wells. The unknown sample concentration can be interpolated from a standard curve generated by the humanized anti-S1RBD monoclonal antibody (mAb).
Intended Uses: SARS-CoV-2 S1RBD IgG ELISA Kit is a highly sensitive and specific immunoassay for the precision detection and quantitative measurement of IgG class antibodies against the S1RBD of SARS-CoV-2 virus in human blood samples.
