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Rabbit Fibrinogen ELISA Kit
Rabbit Fibrinogen ELISA Kit
- 中文名称：
- Rabbit Fibrinogen ELISA Kit
- 英文名称：
- Rabbit Fibrinogen ELISA Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA16031
- 规格：
- 48-Strip-Wells|96-Strip-Wells|2x96-Strip-Wells|3x96-Strip-Wells|4x96-Strip-Wells
- 保存建议：
- 将所有试剂储存在2-8摄氏度
- 货期：
- 6-8周
- 检测种属：
- Rabbit
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
For Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma
Intended Uses: This FBG/SAPK ELISA kit is intended for laboratory research use only. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of FBG/SAPK in the sample, this FBG/SAPK ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus FBG/SAPK concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of FBG/SAPK utilizes a multiclonal anti-FBG/SAPK antibody and an FBG/SAPK-HRP conjugate. The assay sample and buffer are incubated together with FBG/SAPK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FBG/SAPK concentration since FBG/SAPK from samples and FBG/SAPK-HRP conjugate compete for the anti-FBG/SAPK antibody binding site. Since the number of sites is limited, as more sites are occupied by FBG/SAPK from the sample, fewer sites are left to bind FBG/SAPK-HRP conjugate. Standards of known FBG/SAPK concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of FBG/SAPK. The FBG/SAPK concentration in each sample is interpolated from this standard curve.
