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Rat MT (Melatonin) ELISA Kit
Rat MT (Melatonin) ELISA Kit
- 中文名称：
- Rat MT (Melatonin) ELISA Kit
- 英文名称：
- Rat MT (Melatonin) ELISA Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA164661
- 规格：
- 24 Tests (LIMIT 1)|48 Tests|96 Tests|2x96 Tests|3x96 Tests
- 保存建议：
- 储存在4摄氏度
- 货期：
- 6-8周
- 检测种属：
- Rat
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Description: The kit is a competitive enzyme immunoassay for in vitro quantitative measurement of MT in Rat serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Rat MT. During the reaction, Rat MT in the sample or standard competes with a fixed amount of Rat MT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat MT. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Rat MT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
