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Human Cytochrome P450 3A4 ELISA Kit
Human Cytochrome P450 3A4 ELISA Kit
- 中文名称：
- Human Cytochrome P450 3A4 ELISA Kit
- 英文名称：
- Human Cytochrome P450 3A4 ELISA Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA16518
- 规格：
- 48-Strip-Wells-(Competitive)|48-Strip-Wells-(Sandwich)|96-Strip-Wells-(Competitive)|96-Strip-Wells-(Sandwich)|2x96-Strip-Wells-(Competitive)
- 保存建议：
- 将所有试剂储存在2-8摄氏度
- 货期：
- 6-8周
- 检测种属：
- Human
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Principle of the Assay: CYP3A4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CYP3A4. Standards or samples are then added to the microtiter plate wells and CYP3A4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CYP3A4 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CYP3A4 are added to each well to "sandwich" the CYP3A4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CYP3A4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CYP3A4 concentration in each sample is interpolated from this standard curve.
Intended Uses: This CYP3A4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CYP3A4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
