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CS (Chondroitin Sulfate) ELISA Kit
CS (Chondroitin Sulfate) ELISA Kit
- 中文名称：
- CS (Chondroitin Sulfate) ELISA Kit
- 英文名称：
- CS (Chondroitin Sulfate) ELISA Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA172676
- 规格：
- 24 Tests (LIMIT 1)|48 Tests|96 Tests|2x96 Tests|3x96 Tests
- 保存建议：
- 储存在4摄氏度
- 货期：
- 6-8周
- 检测种属：
- General
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Cortisol concentrations in Porcine serum and plasma. Please contact tech-support for other sample type detection.
Principle of the Assay: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Cortisol. During the reaction, Cortisol in the sample or standard competes with a fixed amount of Cortisol on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Cortisol. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Cortisol in the samples is then determined by comparing the OD of the samples to the standard curve!!Background/Introduction: Cortisol is often called the "stress hormone" because of its connection to the stress response, however, cortisol is much more than just a hormone released during stress. Cortisol is one of the steroid hormones and is made in the adrenal glands. Secretion of the hormone is controlled by the hypothalamus, the pituitary gland, and the adrenal gland, a combination glands often referred to as the HPA axis. Because most bodily cells have cortisol receptors, it affects many different functions in the body. Cortisol can help control blood sugar levels, regulate metabolism, [1] help reduce inflammation, and assist with memory formulation. It has a controlling effect on salt and water balance and helps control blood pressure. In women, cortisol also supports the developing fetus during pregnancy. Low cortisol levels can cause a condition known as primary adrenal insufficiency or Addison disease. While rare, primary adrenal insufficiency is an autoimmune disease that causes damage to the adrenal glands Cortisol reduces bone formation, favoring long-term development of osteoporosis. It transports potassium out of cells in exchange for an equal number of sodiumions. This can trigger the hyperkalemia of metabolic shock from surgery. Cortisol also reduces calcium absorption in the intestine [2].
