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Hydroxyproline assay kit (Alkali hydrolysis method)
Hydroxyproline assay kit (Alkali hydrolysis method)
- 中文名称：
- Hydroxyproline assay kit (Alkali hydrolysis method)
- 英文名称：
- Hydroxyproline assay kit (Alkali hydrolysis method)
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA172694
- 规格：
- 50 Assays|100 Assays|2x100 Assays|3x100 Assays|4x100 Assays
- 保存建议：
- Store at 2-8 degree C. Valid for 12 months
- 货期：
- 6-8周
- 检测种属：
- N/A
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Background/Introduction: Hydroxyproline accounts for 13.4% in collagen and very little in elastin, but cannot be found in other proteins. Collagen is mostly distributed in skin, tendon, cartilage, blood vessels, etc. So the amount of hydroxyproline can reflect the collage metabolism of the connective disease. The determination of hydroxyproline in skin is a screening index of anti-aging medicines. The excretion of hydroxyproline will increase with age in development stage, then keep stable after non-development. The hydroxyproline in elderly is less than in the young. When rats suffer from spontaneous hypertension and secondary hypertension, the content of hydroxyproline will increase in their urine. Surgical trauma will result in an increase of hydroxyproline in the urinary excretion. High lipid, low protein, alcoholic poisoning toxicant, malnutrition, or liver cell degeneration and necrosis will lead to the increase of fiber by inflammation which splits liver lobule, leading to cirrhosis of the liver. When the liver fibrosis, the collagen fibers are the mainly increasing composition in liver, which uniquely contains the hydroxyproline. The content of liver hydroxyproline can be converted to the content of the liver collagen, reflecting the degree of liver fibrosis. The decomposition of collagen fiber mainly depends on collagen enzyme and other protease. The hydroxyproline decomposed products will be excreted by the urine. The collagen degradation can be reflected by the content of urinary hydroxyproline. By determining the content of hydroxyproline in the tissue and urine, the degree of fibrosis can be judged, and the preventing and treating drugs can also be screened. This kit can be used to detect the content of hydroxyproline(HYP) in animal serum(plasma),tissue urine etc.
