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Urea (Urease method) assay kit
Urea (Urease method) assay kit
- 中文名称：
- Urea (Urease method) assay kit
- 英文名称：
- Urea (Urease method) assay kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA172731
- 规格：
- 50 Assays|100 Assays|2x100 Assays|3x100 Assays|4x100 Assays
- 保存建议：
- Store at 2-8 degree C. Valid for 12 months
- 货期：
- 6-8周
- 检测种属：
- N/A
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Principle of the Assay: Unlike most cell-based BrdU assay kits that can only measure qualitative differences in BrdU incorporation, the CytoSelect BrdU Competitive ELISA kit allows quantitative measurement of BrdU. The unknown BrdU-containing samples or free BrdU standards are first added to a BrdU conjugate preadsorbed microplate. After a brief incubation, an anti-BrdU monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The BrdU content in unknown samples is determined by comparison with a predetermined BrdU standard curve!!Background/Introduction: Bromodeoxyuridine (BrdU) is a synthetic analog of the natural nucleoside thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues. Introduction of BrdU to cells results in uptake and incorporation into the newly synthesized DNA of replicating cells in place of thymidine. Antibodies specific for BrdU can then be used to detect the incorporated BrdU. The CytoSelect BrdU Competitive ELISA Kit is a competitive enzyme immunoassay developed for rapid detection and quantitation of BrdU in DNA samples. The quantity of BrdU in unknown samples is determined by comparing its absorbance with that of a known BrdU standard curve. The kit has detection sensitivity limit of 80 ng/mL BrdU. Each CytoSelect BrdU Competitive ELISA Kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.
