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Noradrenaline/Norepinephrine ELISA Kit
Noradrenaline/Norepinephrine ELISA Kit
- 中文名称：
- Noradrenaline/Norepinephrine ELISA Kit
- 英文名称：
- Noradrenaline/Norepinephrine ELISA Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA17324
- 规格：
- 48-Strip-Wells|96-Strip-Wells|2x96-Strip-Wells|3x96-Strip-Wells|4x96-Strip-Wells
- 保存建议：
- 将整套工具短期储存在2-8摄氏度的环境中。如果要长期保存，请将微孔板和标准品保存在-20摄氏度，而剩余的试剂可以保存在2-8摄氏度
- 货期：
- 6-8周
- 检测种属：
- General
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Background: Norepinephrine, the scientific name 1-(3, 4-dihydroxyphenyl) -2-aminoethanol, is a substance formed by the removal of n-methyl from epinephrine, and is also a catecholamine in chemical structure. It is both a neurotransmitter, mainly synthesized and secreted by sympathetic postganglionic neurons and norepinephrine neurons in the brain, which is the main transmitter released by the latter, and a hormone, synthesized and secreted by the adrenal medulla, but in smaller amounts. The main source of norepinephrine in the circulating blood is the adrenal medulla.
Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with NA/NE. During the reaction, NA/NE in the sample or standard competes with a fixed amount of NA/NE on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to NA/NE. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzymesubstrate reaction is terminated by the addition of a acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of NA/NE in the samples is then determined by comparing the OD of the samples to the standard curve. The concentration of the target substance was inversely proportional to the OD450 value.
