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Rat Beta-Endorphin ELISA Kit
Rat Beta-Endorphin ELISA Kit
- 中文名称：
- Rat Beta-Endorphin ELISA Kit
- 英文名称：
- Rat Beta-Endorphin ELISA Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA17569
- 规格：
- 48-Strip-Wells|96-Strip-Wells|2x96-Strip-Wells|3x96-Strip-Wells|4x96-Strip-Wells
- 保存建议：
- 将整套工具短期储存在2-8摄氏度的环境中。如果要长期保存，请将微孔板和标准品保存在-20摄氏度，而剩余的试剂可以保存在2-8摄氏度
- 货期：
- 6-8周
- 检测种属：
- Rat
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Background: Beta-endorphin is an endogenous opioid peptide neurotransmitter found in the neurons of both the central and peripheral nervous system. Beta-endorphin is a peptide, 31 amino acids long, resulting from processing of the precursor pro-opiomelanocortin (POMC). Beta-endorphin is found in neurons of the hypothalamus as well as the pituitary gland. It is an agonist of the opioid receptors, with evidence suggesting that it serves as the endogenous ligand of the u-opioid receptor, the same receptor to which the chemicals extracted from opium, such as morphine and codeine, have their analgesic and addictive effects.
Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with beta-EP. During the reaction, beta-EP in the sample or standard competes with a fixed amount of beta- EP on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to beta-EP. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzymesubstrate reaction is terminated by the addition of a acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of beta-EP in the samples is then determined by comparing the OD of the samples to the standard curve. The concentration of the target substance was inversely proportional to the OD450 value.
