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Mouse Neutrophil Elastase ELISA Kit
Mouse Neutrophil Elastase ELISA Kit
- 中文名称：
- Mouse Neutrophil Elastase ELISA Kit
- 英文名称：
- Mouse Neutrophil Elastase ELISA Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA17638
- 规格：
- 48-Strip-Wells|96-Strip-Wells|2x96-Strip-Wells|3x96-Strip-Wells|4x96-Strip-Wells
- 保存建议：
- 将整套工具短期储存在2-8摄氏度的环境中。如果要长期保存，请将微孔板和标准品保存在-20摄氏度，而剩余的试剂可以保存在2-8摄氏度
- 货期：
- 6-8周
- 检测种属：
- Mouse
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Background: Neutrophil Elastase (NE, ELANE), also known as HNE, is a chymotrypsin family serine protease that plays a key role in pathogen clearance. It is expressed by promyelocytes and stored in the intracellular azurophilic granules of polymorphonuclear leukocytes (PMN). These granules fuse with phagosomes, enabling Neutrophil Elastase to participate in the digestion and killing of endocytosed microbes. The enzyme is released by activated neutrophils at sites of inflammation, and it can remain associated with the cell surface or function as a component of neutrophil extracellular nets (NETs) which trap and kill microbial pathogens.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti NE antibody was pre-coated onto the 96-well plate. The biotin conjugated anti NE antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with NE conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of NE in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
