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Mouse Free Thyroxine ELISA Kit
Mouse Free Thyroxine ELISA Kit
- 中文名称：
- Mouse Free Thyroxine ELISA Kit
- 英文名称：
- Mouse Free Thyroxine ELISA Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA17696
- 规格：
- 48-Strip-Wells|96-Strip-Wells|2x96-Strip-Wells|3x96-Strip-Wells|4x96-Strip-Wells
- 保存建议：
- 将整套工具短期储存在2-8摄氏度的环境中。如果要长期保存，请将微孔板和标准品保存在-20摄氏度，而剩余的试剂可以保存在2-8摄氏度
- 货期：
- 6-8周
- 检测种属：
- Mouse
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Background: Thyroxine (sometime called T4) is a hormone produced by the thyroid gland. The term "free thyroxine" means measured thyroxine that is not bound to proteins in the blood. Greater than 99% of thyroxine is bound to serum proteins, but the portion that is not, free thyroxine, is the biologically active portion. In most cases, free thyroxine (also known as free T4) is good indicator of thyroid function, along with TSH and other measures. Free thyroxine is often more useful than total thyroxine in assessing thyroid function.
Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with fT4. During the reaction, fT4 in the sample or standard competes with a fixed amount of fT4 on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to fT4. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of fT4 in the samples is then determined by comparing the OD of the samples to the standard curve. The concentration of the target substance was inversely proportional to the OD450 value.
