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Superoxide Dismutase (SOD) Typed Activity Assay Kit (Hydroxylamine Method)
Superoxide Dismutase (SOD) Typed Activity Assay Kit (Hydroxylamine Method)
- 中文名称：
- Superoxide Dismutase (SOD) Typed Activity Assay Kit (Hydroxylamine Method)
- 英文名称：
- Superoxide Dismutase (SOD) Typed Activity Assay Kit (Hydroxylamine Method)
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA176972
- 规格：
- 48 Tests|96 Tests|2x96 Tests|3x96 Tests|4x96 Tests
- 保存建议：
- Reagent4: -20 degree C; Others: 2-8 degree C. Valid for 12 months.
- 货期：
- 6-8周
- 检测种属：
- General
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Background: According to the literature, superoxide dismutase exists in all oxygenmetabolizing cells to protect cells from excessive superoxide. Under the action of SOD, two superoxide anions were converted to oxygen and hydrogen peroxide.In mammals, there are three different forms of SOD: CuZn-SOD, Mn-SOD and EC-SOD (an extracellular form of SOD). Cu-Zn SOD exists in the cytoplasmic and mitochondrial membrane spaces of the cells, while Mn-SOD is located in the mitochondrial matrix.
Principle of the Assay: Superoxide anion (O2•-) produced by xanthine and xanthine oxidase system can oxidize hydroxylamine to form nitrite which appear purplish red after chromogenic reaction. The SOD in the sample has a specific inhibitory effect on superoxide anion (O2•-), can reduce the content of nitrite. The OD value is lower than control and the activity of SOD is calculated through the formula. There are two kinds of SOD (CuZn-SOD, Mn-SOD) in the cells of higher animals, and the sum of them is equal to the total SOD. The activity of Mn-SOD in the sample will lost after sample pretreatment, but the activity of CuZn-SOD will not.
Intended Uses: This kit can be used to measure T-SOD, CuZn-SOD, Mn-SOD activity in serum, plasma, urine, cells, cell culture supernatant and tissue homogenate samples.
