-
Mouse PGE2 (Prostaglandin E2)
Mouse PGE2 (Prostaglandin E2)
- 中文名称：
- Mouse PGE2 (Prostaglandin E2)
- 英文名称：
- Mouse PGE2 (Prostaglandin E2)
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA17772
- 规格：
- 48-Strip-Wells|96-Strip-Wells|2x96-Strip-Wells|3x96-Strip-Wells|4x96-Strip-Wells
- 保存建议：
- 将整套工具短期储存在2-8摄氏度的环境中。如果要长期保存，请将微孔板和标准品保存在-20摄氏度，而剩余的试剂可以保存在2-8摄氏度
- 货期：
- 6-8周
- 检测种属：
- Mouse
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Background: Prostaglandin E2 (PGE2) is an important bioactive molecule and a member of the prostaglandin family. It is generated from arachidonic acid (AA) by the action of cyclooxygenase (COX). PGE2 is widely present in the Mammals body and can regulate various physiological and pathological processes, such as inflammation, immune response, pain perception, temperature regulation, and modulation of cardiovascular and digestive system function. PGE2 exerts its effects by binding to its four different types of receptors (EP1, EP2, EP3, EP4). Different types of receptors have distinct signal transduction pathways and functions, including regulation of cell proliferation, apoptosis, migration, invasion, cytokine release, and ion channel modulation. PGE2 plays an important role in the occurrence and development of many diseases, including inflammatory diseases, cancer, and cardiovascular diseases. Therefore, PGE2 and its receptors are important targets for researching and developing disease treatments.
Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with PGE2. During the reaction, PGE2 in the sample or standard competes with a fixed amount of PGE2 on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to PGE2. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme- substrate reaction is terminated by the addition of a acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of PGE2 in the samples is then determined by comparing the OD of the samples to the standard curve. The concentration of the target substance was inversely proportional to the OD450 value.
