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Human SARS-CoV-2 Neutralization Antibody ELISA Kit
Human SARS-CoV-2 Neutralization Antibody ELISA Kit
- 中文名称：
- Human SARS-CoV-2 Neutralization Antibody ELISA Kit
- 英文名称：
- Human SARS-CoV-2 Neutralization Antibody ELISA Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA177765
- 规格：
- 24 Tests (LIMIT 1)|48 Tests|96 Tests|2x96 Tests|3x96 Tests
- 保存建议：
- The unopened kit can be stable for 6 months at 2-8 degree C. After opening the kit, keep the reagents according to the conditions on the next page.
- 货期：
- 6-8周
- 检测种属：
- N/A
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Intended Uses: This ELISA kit applies to the in vitro semi-quantitative determination of Neutralization antibodies against SARS-CoV-2 in human serum or plasma.
Principle of the Assay: This Test kit uses Competitive-ELISA as the method to semi-quantitatively detect the Anti-SARS-CoV-2 Neutralization Antibody in the sample. The micro ELISA plate provided in this kit is pre-coated with recombinant human ACE2. During the reaction, the SARS-CoV-2 Neutralization Antibody in the pretreated samples or standards/controls competes with a fixed amount of human ACE2 on the solid phase supporter for sites on the Horseradish peroxidase (HRP) conjugated recombinant SARS-CoV-2 RBD fragment (HRP-RBD). After 37 degree C incubation, the unbound HRP-RBD as well as any HRP-RBD bound to non-Neutralization antibody will be captured on the plate and eventually form the ACE2-RBD-HRP complex, while the circulating neutralization antibodies HRP-RBD complexes remain in the supernatant and are removed during washing. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of SARS-CoV-2 Neutralization Antibody in the samples is then determined by comparing the OD of the samples to the standard curve.
