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Free Cholesterol (FC) Colorimetric Assay Kit
Free Cholesterol (FC) Colorimetric Assay Kit
- 中文名称：
- Free Cholesterol (FC) Colorimetric Assay Kit
- 英文名称：
- Free Cholesterol (FC) Colorimetric Assay Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA177777
- 规格：
- 48 Tests|96 Tests|500 Assays|2x500 Assays|3x500 Assays
- 保存建议：
- Store at 2-8 degree C. Valid for 12 months
- 货期：
- 6-8周
- 检测种属：
- N/A
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Intended Uses: This kit applies the COD-PAP method and it can be used for in vitro determination of free cholesterol content in serum, plasma, tissue samples.
Principle of the Assay: Free cholesterol produces 4-cholestenone and hydrogen peroxide under the oxidation of cholesterol oxidase. In the presence of 4-aminoamylpyridine and phenol, peroxidase catalyze hydrogen peroxide to form red quinone compounds of benzoquinone imine phenizone. The color depth of the generated quinones is directly proportional to the cholesterol content.
Background/Introduction: Cholesterol is a kind of sterol and lipid in cell membrane. Most of cholesterol in blood exists in the form of cholesterol ester. Lecithin-cholesterol acyltransferase in human plasma is an enzyme that catalyzes the formation of cholesterol ester. Cholesterol synthesized or deposited in peripheral cells returns to the liver through the reverse cholesterol transport system for reusing or regaining bile acids.
