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RIPA Lysis Buffer
RIPA Lysis Buffer
- 中文名称：
- RIPA Lysis Buffer
- 英文名称：
- RIPA Lysis Buffer
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA125831
- 规格：
- 100 mL|2x100 mL|3x100 mL|5x100 mL|4x100 mL
- 保存建议：
- Upon receipt store at 4 degree C. RIPA Lysis Buffer is stable for one year. Product is shipped on ice.
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- Liquid. 50mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Description: RIPA Lysis Buffer is a complete cell lysis solution reagent used for rapid and efficient total cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells, effectively extracting cytoplasmic, nuclear and membrane proteins. Protein lysis can be finished in 60 minutes.
Background: RIPA lysis extraction buffer contains non-ionic and ionic detergents which are able to extract protein from wide variety of cell types and membrane structures. RIPA buffer ensures efficient cell lysis and protein solubilization preventing protein degradation and interference with protein immunoreactivity and biological activity. Since most antibodies and protein antigens are not adversely affected by the components of this solution, RIPA buffer-conducted protein extraction is compatible with various downstream immunoprecipitation and molecular pull-down assays, including reporter assays, protein assays, immunoassays and protein purification. RIPA buffer reagent minimizes non-specific protein-binding interactions to keep background low, while allowing most specific interactions to occur, enabling studies of relevant protein-protein interactions.
