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DiTag MMAE IgG labeling reagent
DiTag MMAE IgG labeling reagent
- 中文名称：
- DiTag MMAE IgG labeling reagent
- 英文名称：
- DiTag MMAE IgG labeling reagent
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA129417
- 规格：
- 0.01 mg|0.05 mg|0.1 mg|2x0.1 mg|3x0.1 mg
- 保存建议：
- The reagents are supplied in lyophilized form. We recommend storing the vial (s) at -20?, desiccated and protected from light. Once reconstituted, the reagents can be stored at 2-8? for 1~2 weeks, or with 50% glycerol at -20?.
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
DiTag MMAE IgG labeling reagents provide an easy solution for quantifying antibody internalization activities. Leveraging VcMMAE (MC-VC-PABC-MMAE) conjugated to an Fc binding protein, these reagents bind to IgG antibodies from various species, resulting in the formation of a VcMMAE-labeled antibody-reagent complex. Upon antibody internalization, the cleavable linker MC-VC-PABC is enzymatically cleaved by cathepsin B, a protein overexpressed in multiple cancer types. This enzymatic cleavage triggers the release of PABC-substituted MMAE, forming an unstable intermediate that liberates the free drug. Measurement of cell killing or inhibition allows researchers to evaluate the efficiency of antibody internalization into cells. This critical information enhances our understanding of the cellular uptake mechanism of antibodies and aids in assessing their efficacy in targeted therapies or test applications.
