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TthPlus-Taq DNA Polymerase
TthPlus-Taq DNA Polymerase
- 中文名称：
- TthPlus-Taq DNA Polymerase
- 英文名称：
- TthPlus-Taq DNA Polymerase
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA13740
- 规格：
- 500 Units|2x500 Units|3x500 Units|4x500 Units|5x500 Units
- 保存建议：
- Store TthPlus DNA polymerase preferably at -20°C in a constant temperature freezer.
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- 10 mM K-phosphate buffer pH 7.0 (25°C), 100 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 50% glycerol (v/v), 0,1 mg/ml BSA.
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
TthPlus DNA polymerase is isolated from the Thermus thermophilus strain. TthPlus DNA polymerase is a single 92 kDa polypeptide showing a 5'-3' exonuclease activity but lacking 3'-5' exonuclease activity. It catalyzes the polymerization of nucleotides into double-stranded DNA in the presence of MgCl2. Its efficiency has been shown more particularly on large DNA fragments up to 12 kb (using lambda phage DNA as a template). TthPlus DNA polymerase is also capable of catalyzing the polymerization of DNA using a RNA template in the presence of Mn2+. The ability of TthPlus DNA polymerase to reverse transcribe at elevated temperatures (70°C) minimizes the problems encountered with strong secondary structures in RNA since they are unstable at higher reaction temperatures. Higher temperatures also result in increased specificity of primer hybridization and extension. In coupled RT/PCR assays, TthPlus is about 50-100 times more efficient than regular Taq DNA polymerase.
TthPlus DNA polymerase is delivered with a one-tube 10x buffer. Both reactions (RT and PCR) are carried out in the same buffer and same tube with the same enzyme (TthPlus). The 10 x one tube buffer does not contain Mn(OAc). Mn(OAc) is provided extra and have to be added at a final concentration of 3.5 mM.
