-
SARS-CoV-2 Pseudoviral Particles, 614G
SARS-CoV-2 Pseudoviral Particles, 614G
- 中文名称：
- SARS-CoV-2 Pseudoviral Particles, 614G
- 英文名称：
- SARS-CoV-2 Pseudoviral Particles, 614G
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA13784
- 规格：
- 5 mL|2x5 mL|3x5 mL|4x5 mL|5x5 mL
- 保存建议：
- Upon receipt, store at -70 degree C right away. Thaw before immediate use.
*Note: read the instruction for thawing in the following protocol carefullly. Do not aliquot and refreeze.
Shelf Life: Six months from date of receipt when stored at -70 degree C.
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- N/A
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
It has been known that SARS coronavirus 2 (SARS-CoV-2) uses human ACE2 as the entry receptor and human proteases as the entry activators. The virus surface spike (S) protein mediates SARS-CoV-2 entry into cells. To fulfill its function, SARS-CoV-2 spike binds to the receptor human ACE2 (hACE2) through its receptor-binding domain (RBD) and is proteolytically activated by human proteases.
Our SARS-CoV-2 Pseudoviral Particles are replication-deficient MLV pseudotyped with the SARS-CoV-2 spike protein. They also contain the ORF for firefly luciferase as a reporter. They establish a pseudovirus entry assay for SARS-CoV-2 as the S protein mediated cell entry can be conveniently measured via the luciferase reporter activity. This pseudovirus assay isolates the SARS-CoV-2 viral entry from other steps of the viral infection cycle.
A new SARS-CoV-2 strain with an amino acid change at position 614 from Asp to Gly in the viral S protein predominated over time in locales where it was found. It is reported by the Choe lab in The Scripps Research Institute that this currently dominate new strain with the D614G variation in the S protein “reduces S1 shedding and increases infectivity”. To support the study of this new SARS-CoV-2 strain, we established the SARS-CoV-2-614G Pseudoviral Particles with the spike protein carrying the 614G genotype (Genbank Accession #YP_009724390.1, p.614D->G)
