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Annexin V-AF647/7-AAD Apoptosis Detection Kit
Annexin V-AF647/7-AAD Apoptosis Detection Kit
- 中文名称：
- Annexin V-AF647/7-AAD Apoptosis Detection Kit
- 英文名称：
- Annexin V-AF647/7-AAD Apoptosis Detection Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA174683
- 规格：
- 20 Assays|50 Assays|100 Assays|200 Assays|2x200 Assays
- 保存建议：
- Annexin V-AF647 and 7-AAD Viability Staining Solution should be stored in dark. 7-AAD Viability Staining Solution should be split into small tubes and storage at -20°C. Other reagents should be stored at 4 °C.
Cautions:
1. For maximal assay performance, this kit should be used within 3-6 months. Avoid freeze/thaw cycles.
2. Detect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.
3. Avoid extended exposure of the samples to direct light to protect the fluorophores from quenching.
4. For your safety and health, please wear the lab coat and disposable gloves before the experiments.
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- N/A
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Annexin V-AF647/7-AAD Cell Apoptosis Detection kit is developed to identify apoptotic and necrotic cells.
Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The fluorescent format of this intracellular protein, Annexin V-AF647, binds specifically to the PS on outer leaflet of cell membrane by flow cytometry or fluorescence microscopy.
7-AAD (7-amino-actinomycin D) has a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. Due to the loss of integrity of membrane, 7-AAD can enter late apoptotic or necrotic cells to stain DNA. Cells at different apoptotic stages can be distinguished by using Annexin V and 7-AAD.
