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0.25% Trypsin Solution
0.25% Trypsin Solution
- 中文名称：
- 0.25% Trypsin Solution
- 英文名称：
- 0.25% Trypsin Solution
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA174717
- 规格：
- 125 mL|500 mL|2x500 mL|3x500 mL|4x500 mL
- 保存建议：
- Store at -20 degree C, shading light.
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- Liquid
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Trypsin is a serine hydrolase that can cut the base-side segment of the lysine and arginine residue in the polypeptide chain, hydrolyze the protein between the cells, and destroy the connection between the cells, so that the tissue or the adherent cells are dispersed into a single cell. The activity of the trypsin is related to the characteristics of the tissue or the cells, the concentration of the trypsin, the temperature and the time of action. At pH 8.0 and 37°C, the effect of the trypsin is the best. Therefore, the concentration of trypsin, the temperature and the time of the pancreatic enzyme should be grasped to avoid the excessive damage of the cells.
In general, the frequently-used working concentration of the trypsin is 0. 25%, while the concentration of trypsin for the semi-adherent cells or the cells is 0.05%. Since EDTA is able to bind Ca2+ and Mg2+ to destroy the cell connection to promote the dissociation of the cells, a certain amount of EDTA is often added to the trypsin solution to enhance the dissociation effect.
