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Calcein-AM/PI Double Staining Kit
Calcein-AM/PI Double Staining Kit
- 中文名称：
- Calcein-AM/PI Double Staining Kit
- 英文名称：
- Calcein-AM/PI Double Staining Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA178139
- 规格：
- 100 Assays|500 Assays|2000 Assays|2x2000 Assays|3x2000 Assays
- 保存建议：
- Store at -20 degree C, shading light
Estimated Expiration: 12 months
Shipping: Ice bag
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- PBS (pH7.2~7.6)(E-IR-R187).
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Background: Calcein AM/PI Double Staining Kit can be used to distinguish dead cells and living cells in mammals with esterase activity. Calcein AM is the addition of acetyl methoxy methyl ester (AM) group to traditional Calcein, which increases hydrophobicity and can easily penetrate the living cell membrane and enter the cell. Calcein AM itself has no fluorescence. After entering the cell, it is hydrolyzed by endogenous esterase in the cell to produce Calcein, a polar molecule with strong negative charge and cannot pass through the cell membrane, while Calcein can emit strong green fluorescence (Ex/Em = 494nm/517nm). Due to the lack of esterase, dead cells cannot or rarely produce Calcein, so only living cells are stained with strong green fluorescence, and dead cells cannot be stained or stained very weakly. The selective membrane permeability of dead cells is lost, and Propidium Iodide (PI) can enter the cell to specifically bind to double-stranded DNA and produce strong red fluorescence(Ex/Em = 535nm/617nm) to label dead cells. Therefore, the combination of Calcein AM and PI can perform double fluorescence staining on living cells and dead cells at the same time, which can be used for the detection of cell activity and cytotoxicity.
