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One-step TUNEL Flow Cytometry Apoptosis Kit (Red, AF555)
One-step TUNEL Flow Cytometry Apoptosis Kit (Red, AF555)
- 中文名称：
- One-step TUNEL Flow Cytometry Apoptosis Kit (Red, AF555)
- 英文名称：
- One-step TUNEL Flow Cytometry Apoptosis Kit (Red, AF555)
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA178150
- 规格：
- 20 Assays|50 Assays|100 Assays|2x100 Assays|3x100 Assays
- 保存建议：
- Store at -20 degree C, shading light
Estimated Expiration: 12 months
Shipping: Ice bag
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- PBS buffer (with 1%BSA) (PH7.2 ~ 7.4)
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Background: One-step TUNEL Flow Cytometry Apoptosis Kit applies a highly sensitive, fast and simple method to detect cell apoptosis. This kit can be used to detect apoptosis of suspension cells and adherent cells by flow cytometry.
Principle of the Assay: When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. After fixation and permeabilization, the exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, and detected by flow cytometry.
