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ca2+ fluorescent probe (fluo-3)
ca2+ fluorescent probe (fluo-3)
- 中文名称：
- ca2+ fluorescent probe (fluo-3)
- 英文名称：
- ca2+ fluorescent probe (fluo-3)
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA269816
- 规格：
- 100 Tests|2x100 Tests|3x100 Tests|4x100 Tests|
- 保存建议：
- 请按照说明书指示
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- N/A
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
This probe is suitable for detecting the intracellular calcium ion concentration (Ca2+Flow) change, is also often used in 96 or 384 wells GPCR high throughput drug screening.
Calcium ion Ca2+ indicated fluorescent probe Fluo series with Ca2+ in combination with the fluorescence enhancement, it is mainly used to detect all aspects of calcium ion signaling pathway, namely calcium flow, such as pharmacological screening at the cellular level, second messenger, neurotransmitter, photoactivation of chelator, etc.
Fluo-2 was one of the first Fluo series of calcium ion indicators invented by Dr. Roger Tsien. Fluo-2 is brighter in cells than Fluo-3 and Fluo-4, mainly probably due to the higher capacity of most cells to load Fluo-2 than Fluo-3 and Fluo-4. Therefore, Fluo-2 is often used for intracellular calcium ion imaging. Fluo-3 is often used on flow cytometry analysis because it can be excited by an argon ion laser 488nmand after binding with Ca2+, a very bright fluorescent signal is emitted.
Fluo-4 is an analogue of Fluo-3 replacing chlorine atoms with two fluorine atoms. This small structural change enhances the fluorescence signal of Fluo-4 probe at 488nm excitation light. The signal is stable and can be applied to confocal microscopy, flow cytometer, fluorescence luminometer / plate reader.
