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SARS-CoV-2 Spike RBD Nanobody
SARS-CoV-2 Spike RBD Nanobody
- 中文名称：
- SARS-CoV-2 Spike RBD Nanobody
- 英文名称：
- SARS-CoV-2 Spike RBD Nanobody
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA27051
- 规格：
- 0.05 mL|0.1 mL|2x0.1 mL|3x0.1 mL|4x0.1 mL
- 保存建议：
- 收到后，储存在-20摄氏度或-80摄氏度。避免反复冷冻。
- 货期：
- 6-8周
- 纯度：
- Affinity-chromatography
- 产品形式：
- Liquid
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
This SARS-CoV-2 S1-RBD (Spike Glycoprotein S1 receptor-binding domain) antibody is a recombinant monoclonal antibody (also a Nanobody) generated through the expression of a DNA sequence inserting a human IgG1 Fc domain at the C-terminus, in human embryonic kidney 293 cells (HEK293). The DNA sequence encodes the SARS-CoV-2 spike RBD. The antibody is purified by protein G in vitro. It has been validated with high reactivity towards SARS-CoV-2 S1-RBD by a functional ELISA and good sensitivity for human SARS-CoV-2 spike glycoprotein (S protein) via the Colloidal Gold ImmunochromatographyAssay (GICA).
It is also validated in Neutralizing and LSPR. In neu assay, the binding signal of the SARS-CoV-2 S1 RBD antibody was inhibited by ACE2 protein-HRP conjugated inhibitor, with a 0.1074 mg/ml IC50. In LSPR assay, the SARS-CoV-2 S1 RBD antibody showed a high affinity with SARS-CoV-2 Spike protein RBD (affinity constant: 28.2nM).
Specifically binding and recognizing the RBD of the SARS-CoV-2 spike glycoprotein, the SARS-CoV-2 S1 RBD antibody can react with samples infected with human coronavirus SARS-CoV-2. But it does not respond to MERS or SARS-CoV spike protein. Akin to other nanobodies, this recombinant nanobody is small and stable, which allows for its reaching to hidden epitopes such as crevices of target proteins.
