-
USP5 Protein, Human, Recombinant (His Tag)
USP5 Protein, Human, Recombinant (His Tag)
- 中文名称：
- USP5 Protein, Human, Recombinant (His Tag)
- 英文名称：
- USP5 Protein, Human, Recombinant (His Tag)
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA27763
- 规格：
- 0.1 mg|2x0.1 mg|3x0.1 mg|4x0.1 mg|5x0.1 mg
- 保存建议：
- Samples are stable for up to twelve months from date of receipt at -70°C.
Store it under sterile conditions at -20°C to -80°C upon receiving.
Recommend to aliquot the protein into smaller quantities for optimal storage.
Avoid repeated freeze-thaw cycles.
- 货期：
- 6-8周
- 纯度：
- >90% as determined by SDS-PAGE
- 产品形式：
- Lyophilized from sterile 20mM Tris, 150mM NaCl, 10% Glycerol, pH 7.5, 5%Trehalose, 5%Mannitol, 0.01% Tween-80
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Background: Ubiquitin carboxyl-terminal hydrolase 5, also known as Deubiquitinating enzyme 5, Isopeptidase T, Ubiquitin thiolesterase 5, Ubiquitin-specific-processing protease 5, ISOT and USP5, is a member of the peptidase C19 family. USP5 contains 2 UBA domains and one UBP-type zinc finger. The UBP-type zinc finger domain interacts selectively with an unmodified C-terminus of the proximal ubiquitin. Both UBA domains are involved in polyubiquitin recognition. The UBP-type zinc finger domain crystallizes as a dimer linked by a disulfide bond between the Cys-195 residues of both molecules, but there is no evidence that the full-length USP5 exists as a dimer. USP5 cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. USP5 is involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. It binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.
