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TUNEL Apoptosis Detection Kit (Green Fluorescence)
TUNEL Apoptosis Detection Kit (Green Fluorescence)
- 中文名称：
- TUNEL Apoptosis Detection Kit (Green Fluorescence)
- 英文名称：
- TUNEL Apoptosis Detection Kit (Green Fluorescence)
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA330133
- 规格：
- 50 Tests|100 Tests|2x100 Tests|3x100 Tests|4x100 Tests
- 保存建议：
- Stable for at least 6 months at -20 degree C from date of shipment. Once opened, refer to list of materials supplied for storage conditions of individual components.
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- N/A
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Principle of the Assay: When cells undergo apoptosis, endonuclease enzymes are activated to cleave the genomic DNA between nucleosomes. When genomic DNA is cleaved, the exposed 3'-OH can be added with a fluorescein dUTP (FITC12-dUTP) due to the catalysis of Terminal Deoxynucleotidyl Transferase (TdT), apoptosis cells can be detected by fluorescence microscopy. This is the principle of TUNEL (TdT-mediated dUTP Nick-End Labeling) for detecting apoptosis. The Enhanced Fluorescein Mix in the kit contains FITC-12-dUTP and fluorescence enhancement factor. The fluorescence enhancement factor can bind to FITC and enhance the stability amplifies of the signal, which makes the fluorescence brighter and has stronger anti-quenching ability
Background/Introduction: The TUNEL Apoptosis Assay Kit is a highly sensitive, rapid and simple method for cell apoptosis detection. The result can be detected by fluorescence microscopy. This kit is developed to detect tissue (Paraffin embedding, freezing and ultrathin section) and cells (Cell smears, cell climbing smears) in situ apoptotic detection.
